MHC I Monomers and Tetramers
(Major Histocompatibility Complex Class I)

Broad Portfolio of MHC I Alleles and MHC/Peptide Complexes

Immunitrack has a broad portfolio of MHC alleles, including: 65 Human MHC I alleles (subtypes HLA-A, HLA-B, HLA-C), 5 Murine MHC I alleles (MLA) and 1 canine MHC I allele (DLA).

We can produce and deliver custom complexes based on the MHC alleles we have in our repository or based on other alleles that you wish to request. MHC/peptide binding assays will initially be performed to test whether or not we can produce the desired complexes since prediction tools are not always reliable.

MHC I monomers are biotinylated and can be complexed with the labelled streptavidin of your choice. MHC I tetramers are labelled with phycoerythrin (PE) or allophycocyanin (APC), and are ready for use in flow cytometry.

Applications

• Tetramer staining. MHC-peptide tetramers are used to detect antigen-specifc T cells in T cell mixtures using flow cytometry.
• Multimer production. Our monomers are compatible with dextramer, tetramer, pentamer and other multimer technologies.
• T cell stimulation. MHC I molecules in complex with peptide epitopes can be used to stimulate CD8 T cells for flow cytometry experiments.
• Generation of T cell receptor-like antibodies. Peptide/MHC (pMHC) complexes can be used with phage display technology to generate libraries of specific T cell receptor-like antibodies for use in research, diagnosis, and immunotherapy.
• Isolating T cells for T cell receptor-based therapy including CAR-T. Peptide/MHC (pMHC) complexes can be used to isolate and sequence T cell receptors for use in research, diagnosis, and immunotherapy.

Product Information

• Storage Buffer: PBS, 50% glycerol.
• Concentration: 0.5 mg/mL. Determined by absorbance at 280 nm and Coomassie staining.
• Sizes Available: 50, 100, 150, 200, 250, 300, 350, 400 and 450 μg supplied as individual vials containing 100 μL product.
• Sizes Available – custom orders: We can accommodate most requests concerning product size. For A0201, we may produce up to mg quantities.
• Stabilisers: 1 % BSA.
• Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use.
• Expiration: 6 months from date of opening.

Delivery Information

All products are shipped preservative-free in liquid form at 0 °C.

Application Notes

In this application note tetramers for Flu, CMV and Mart1 are tested by flow cytometry on PBMC from blood donors. Download ▸

Protocol for tetramer staining using our tetramers. Download ▸

Supporting Data for MHC Class I Monomers and Tetramers

Our biotinylated MHC I complexes have been used as tetramer reagents as well as for the stimulation of CD8 T cells in several publications. In the example shown below, two tetramers B*0801-IE188-96 and B*0801-IE199-207 were used in flow cytometry experiments to validate CD8 T cell responses against human cytomegalovirus (HCMV) protein IE1.

Source: Adapted from: PlosOne April 2014, Volume 9,Issue 4,e94892 Identification and HLA-Tetramer-Validation of Human CD4+ and CD8+ T Cell Responses against HCMV Proteins IE1 and IE2.

Key Publications

1. Fadel, T.R. et al. A carbon nanotube-polymer composite for T-cell therapy. Nat Nanotechnol. 2014 9(8):639-47.
2. Braendstrup, P. et al. Identification and HLA-tetramer-validation of human CD4+ and CD8+ T cell responses against HCMV proteins IE1 and IE2. PLoS One. 2014. 9(4):e94892.

• Go to full list of MHC I Publications ▸