Immunitrack’s Major Histocompatibility Complex Class II: MHC II

Advantages at a Glance

  • Optimized manufacturing platform
  • Widest range of available HLA alleles
  • Market-leading turnaround time on catalog products
  • 100% peptide loading of MHC complexes
  • Available as MHC-PE tetramers or monomers

Optimized Manufacturing Platform

MHC II is known as challenging molecule to manufacture. In the past, this has led to limited use of MHC II molecules as reagents. However, at Immunitrack we have learned how to consistently and reproducibly manufacture MHC II – peptide complexes.

All MHC manufacturing at Immunitrack is supervised by Sune Justesen, our CSO. Sune has more than 12 years of experience in the manufacture of MHC molecules. This began when Sune was responsible for the development of one of the largest in vitro assay platforms for measuring peptide binding to MHC. Over 100,000 Kd measurements from this project are available today as affinity measurements in NetMHC and the Immune Epitope Database (IEDB).

Widest range of available HLA alleles

We are continuously expanding our MHC II repertoire. Currently we are able to deliver assays or complexes on the following alleles. MHCI alleles not present on the list may be synthesized and produced upon request.

Human  Murine
DRDQDP
DRB1*0101DQA1*0101DPA1*0103I-Ab
DRB1*0301DQB1*0301DPA1*0202I-Ed
DRB1*0401DQB1*0501DPB1*0401I-Ad
DRB1*0701DPB1*0402
DRB1*1101
DRB1*1501
DRB3*0101
DRB3*0202
DRB4*0101
DRB5*0101

Fast Turnaround on Custom Orders

Our MHC II catalog offers a wide range of commonly-requested MHC-peptide complexes. However, many researchers want unique peptide sequences (“non-catalog”). We offer a market-leading turnaround time of 3-5 weeks on orders of custom pMHC complexes.

Times may be shortened for user-supplied peptides, if they are of sufficient quality.

100% Peptide Loading

Our manufacturing process means that you get pMHC with 100% peptide loading of each complex guaranteed. Some techniques for peptide loading of MHC lead to less than 100% occupancy. Our manufacturing and QC guarantee nothing less than 100% peptide loading of your MHC.

Available as MHC-PE tetramers or monomers

At Immunitrack, we offer our pMHC complexes as tetramers (i.e. complexed with streptavidin), or as monomers. Our tetramers are labeled with phycoeryrthin (PE), ready for use in flow cytometry. Our monomers are biotinylated and can be complexed with the labeled streptavidin of your choice.

More Information

Our biotinylated MHC II complexes have been used as tetramer reagents in several publications.

PBMCs were incubated with PE- and APC-conjugated HLA-DRB5*01:01- IE1211-225(h6) tetramers. Double positive cells were cultured for 14 days with irradiated autologous dendritic cells pulsed with IE1211-225 and subsequently tetramer analyzed again with relevant and irrelevant tetramer. Adapted from PLoS One. 2013 Sep. 2;8(9):e73648. doi: 10.1371/journal.pone.0073648

Selected Publications

1.
A carbon nanotube-polymer composite for T-cell therapy.
Nat Nanotechnol. 2014 Aug;9(8):639-47
PMID: 25086604

2.
Identification and HLA-tetramer-validation of human CD4+ and CD8+ T cell responses against HCMV proteins IE1 and IE2.
PLoS One. 2014 Apr 23;9(4):e94892.
PMID: 24760079

3.
Adsorption of multimeric T cell antigens on carbon nanotubes: effect on protein structure and antigen-specific T cell stimulation.
Small. 2013 Mar 11;9(5):666-72.
PMID: 23090793

4.
MHC class II tetramers made from isolated recombinantαandβchains refolded with affinity-tagged peptides.
PLoS One. 2013 Sep 2;8(9):e73648.
PMID: 24023895

5.
Chaperone-assisted thermostability engineering of a soluble T cell receptor using phage display.
Sci Rep. 2013;3:1162.
PMID: 23362461

6.
Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides.
Immunology. 2011 Apr;132(4):482-91.
PMID: 21294723

7.
NetMHCIIpan-2.0 – Improved pan-specific HLA-DR predictions using a novel concurrent alignment and weight optimization training procedure.
Immunome Res. 2010 Nov 13;6:9

8.
HLA class I binding 9mer peptides from influenza A virus induce CD4 T cell responses.
PLoS One. 2010 May 7;5(5):e10533.
PMID: 20479886

9.
Functional recombinant MHC class II molecules and high-throughput peptide-binding assays.
Immunome Res. 2009 May 5;5:2.
PMID: 19416502

10.
Quantitative predictions of peptide binding to any HLA-DR molecule of known sequence: NetMHCIIpan.
PLoS Comput Biol. 2008 Jul 4;4(7):e1000107.
PMID: 18604266