Immunitrack’s Major Histocompatibility Complex Class I: MHC I

Advantages at a Glance

  • Optimized manufacturing platform
  • Widest range of available HLA alleles
  • Market-leading turnaround time on catalog products
  • 100% peptide loading of MHC complexes
  • Available as MHC-PE tetramers or monomers

Optimized Manufacturing Platform

All MHC manufacturing at Immunitrack is supervised by Sune Justesen, our CSO. Sune has more than 12 years of experience in the manufacture of MHC molecules, beginning when Sune was responsible for the development of one of the largest in vitro assay platforms for measuring peptide binding to MHC. Over 100,000 Kd measurements from this project are available today as affinity measurements in NetMHC and the Immune Epitope Database (IEDB).

Widest Range of Available HLA Alleles

Below is a list of currently available MHC I alleles. MHCI alleles not present on the list may be synthesized and produced upon request.

 Human  Murine
HLA-AHLA-BHLA-CKb
A*01:01B*07:02C*04:01Db
A*02:01B*08:01C*07:01Kd
A*02:01B*14:02C*07:02Ld
A*02:06B*15:01Dd
A*02:07B*18:01
A*03:01B*35:01
A*11:01B*42:02
A*24:02B*44:03
A*23:01B*52:01
A*25:01
A*31:01
A*74:01

Fast Turnaround on Custom Orders

Our MHC I catalog offers a wide range of commonly-requested MHC-peptide complexes. However, many researchers want unique peptide sequences (“non-catalog”). We offer a market-leading turnaround time of 3-5 weeks on orders of custom pMHC complexes.

Times may be shortened for user-supplied peptides, if they are of sufficient quality.

100% Peptide Loading

Our manufacturing process means that you get pMHC with 100% peptide loading of each complex guaranteed. Some techniques for peptide loading of MHC lead to less than 100% occupancy. Our manufacturing and QC guarantee nothing less than 100% peptide loading of your MHC.

Available as MHC-PE tetramers or monomers

At Immunitrack, we offer our pMHC complexes as tetramers (i.e. complexed with streptavidin), or as monomers. Our tetramers are labeled with phycoeryrthin (PE), ready for use in flow cytometry. Our monomers are biotinylated and can be complexed with the labeled streptavidin of your choice.

More Information

Our biotinylated MHC I complexes have been used as tetramer reagents as well as for the stimulation of CD8 T cells in several publications.

B*0801 Tetramer staining of two CD8 T cells specific for peptides derived from HCMV Protein IE1. Adapted from: PlosOne April 2014, Volume 9,Issue 4,e94892 Identification and HLA-Tetramer-Validation of Human CD4+ and CD8+ T Cell Responses against HCMV Proteins IE1 and IE2

Selected Publications

Publications where the MHCI were used

1.
Mismatch-repair deficiency predicts response of solid tumors to PD-1 blockade
Science, 2017 Jul 28;357(6349):409-413
PMID: 28596308

2.
Configuration-dependent presentation of multivalent IL-15:IL-15Rα enhances the antigen-specific T cell response and anti-tumor immunity.
J Biol Chem. 2015 Dec 30. pii: jbc.M115.695304.
PMID: 26719339

3.
A carbon nanotube-polymer composite for T-cell therapy.
Nat Nanotechnol. 2014 Aug;9(8):639-47.
PMID: 25086604

4.Identification and HLA-tetramer-validation of human CD4+ and CD8+ T cell responses against HCMV proteins IE1 and IE2.
PLoS One. 2014 Apr 23;9(4):e94892. doi: 10.1371/journal.pone.0094892. eCollection 2014.
PMID: 24760079

5.
Adsorption of multimeric T cell antigens on carbon nanotubes: effect on protein structure and antigen-specific T cell stimulation.
Small. 2013 Mar 11;9(5):666-72. doi: 10.1002/smll.201201684. Epub 2012 Oct 23.
PMID: 23090793

6.
Wildtype p53-specific antibody and T-cell responses in cancer patients.
J Immunother. 2011 Nov-Dec;34(9):629-40. doi: 10.1097/CJI.0b013e3182281381.
PMID: 21989411

7.
Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides.
Immunology. 2011 Apr;132(4):482-91. doi: 10.1111/j.1365-2567.2010.03383.x. Epub 2011 Feb 7.
PMID: 21294723

8.
HLA class I binding 9mer peptides from influenza A virus induce CD4 T cell responses.
PLoS One. 2010 May 7;5(5):e10533. doi: 10.1371/journal.pone.0010533.
PMID: 20479886

9.
Peptide binding to HLA class I molecules: homogenous, high-throughput screening, and affinity assays.
J Biomol Screen. 2009 Feb;14(2):173-80. doi: 10.1177/1087057108329453. Epub 2009 Feb 4.
PMID: 19196700

10.
One-pot, mix-and-read peptide-MHC tetramers.
PLoS One. 2008 Feb 27;3(2):e1678. doi: 10.1371/journal.pone.0001678.
PMID: 18301755

11.
NetMHCpan, a method for quantitative predictions of peptide binding to any HLA-A and -B locus protein of known sequence.
PLoS One. 2007 Aug 29;2(8):e796.
PMID: 17726526

12.
Definition of supertypes for HLA molecules using clustering of specificity matrices.
Immunogenetics. 2004 Mar;55(12):797-810. Epub 2004 Feb 13.
PMID: 14963618